发明名称 |
Pseudomonas putida amidase polypeptide useful for the production of chiral amides and acids |
摘要 |
The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.
|
申请公布号 |
US6251650(B1) |
申请公布日期 |
2001.06.26 |
申请号 |
US20000687594 |
申请日期 |
2000.10.13 |
申请人 |
E. I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
FALLON ROBERT DONALD;NELSON MARK JAMES;PAYNE MARK SCOTT |
分类号 |
C12N15/09;C07K14/21;C12N1/15;C12N1/19;C12N1/21;C12N9/78;C12N9/80;C12N15/00;C12N15/55;C12P7/40;C12P13/02;C12P17/00;C12P41/00;C12R1/19;C12R1/40;C12R1/84;(IPC1-7):C12N9/78 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|