发明名称 |
Protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof |
摘要 |
A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.
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申请公布号 |
US6251651(B1) |
申请公布日期 |
2001.06.26 |
申请号 |
US19990324910 |
申请日期 |
1999.06.03 |
申请人 |
AMANO PHARMACEUTICAL CO., LTD. |
发明人 |
YAMAGUCHI SHOTARO;MATSUURA AKIRA |
分类号 |
A23J3/34;C12N1/21;C12N9/80;C12N15/55;(IPC1-7):C12N9/00;C12N9/50;C12N9/52;C12N9/78 |
主分类号 |
A23J3/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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