| 摘要 |
The present invention relates to complementing cell lines for the production of replication incompetent viruses, which significantly reduce or eliminate the presence of replication competent viruses. Methods to make and use the complementing cell lines are also provided, as are nucleic acid molecules, polynucleotides, and vectors for making the cell lines. In particular, the present invention relates to complementing cell lines for the production of replication incompetent adenoviruses (Ad), which significantly reduce or eliminate the presence of replication competent Ad (RCA) and can serve for t he large scale production of infectious replication incompetent adenovirus particles that may be used for the treatment of human patients as for exampl e in gene therapy. The present invention further relates to an assay for detecting the presence of replication competent virus particles, in particul ar RCA, in a stock of infectious replication incompetent virus particles, in particular replication incompetent adenovirus particles, which employs a rea l time quantitative PCR assay with a sensitivity level to detect one replicati on competent virus particle per >= 109 replication incompetent virus particl es.
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