摘要 |
PURPOSE: A method for analyzing a homocysteine concentration in blood is provided which is excellent in sensitivity, improvement of reproductivity, accuracy, shortening of analysis time, low cost and conveniency. CONSTITUTION: The method comprises steps of: (i) reacting homocysteine with ethyl chloroformate and 2,2,3,3,3-pentafluoro-1-propanol to prepare a derivative of homocysteine; (ii) extracting the homocysteine derivative with an organic solvent; and (iii) analyzing the clean-up homocysteine derivative by using a gas chromatography-electron capture detector.
|