ADENOVIRUS VECTORS FOR THE TRANSFER OF GENES IN TARGETED CELLS

摘要

The invention relates to a Canine Adenovirus (CAV) vector obtainable by a process comprising the following steps: a) co-transforming E. coli cells having recBC sbcBC phenotype by a first plasmid and a pre-transfer plasmid i n conditions enabling their recombination by homologous recombination, in orde r to generate a transfer plasmid devoid from a functional E1 coding region, comprising the desired recombinant vector genome, wherein the first plasmid comprises the Inverted Terminal Regions (ITR) and the Packaging Signal (.psi .) sequences of a CAV genome, and the pre-transfer plasmid includes the sequenc e whose insertion in the vector genome is desired, flanked by sequences homologous to sequences of the first plasmid surrounding the region of the first plasmid where the modification is desired; b) isolating a DNA fragment essentially comprising the recombinant vector genome by enzyme restriction; c) transfecting DK28Cre cells (CNCM I-2293) that are rendered able to transcomplement this recombinant vector genome; d) recovering and purifying the recombinant adenoviral particles produced. The obtained vector may be us ed for the transfer of any gene of interest in target cells, especially in neuronal cells.