| 摘要 |
<p>The invention relates to the field of molecular biology, molecular genetics, and biotechnology and can be used for purposes of genotherapy as well as in agriculture, industrial biotechnology for-gene specific silencing of genes whose expression promotes the development of different diseases and infringes upon a process for producing a required product. The invention proposes a method for modifying the genetic characteristics of organisms by means of RNA interference resulting in the gene-specific silencing of the selected gene with the aid of RNA molecules. The molecules are synthesised in vitro using the RNA molecules which are complementary in a parallel direction with (pcRNA) mRNA of the selected gene which are synthesised in vivo or in vitro on an artificial sequences of DNA which possesses a mirror-symmetrical sequence of nucleotides in respect to the sequence of the gene nucleotides. The invention provides a general approach to the modification of the genetic characteristics of an organism based on the biological characteristics of mirror-inverses of the nucleotide sequences being achieved through RNA-interference which brings about gene-specific silencing.</p> |
