| 摘要 |
<p>The present invention provides immunogens for generating anti-neonicotinoid antibodies as well as antibodies, methods, reagents, and kits for determining the presence of one or more neonicotinoid insecticides in a sample by immunoassay. The present invention has particular application to determination of the concentration of neonicotinoid insecticides applied to a plant propagation materials such as seed, said antibody is selective for a compound of formula (I) wherein A is 2-chloropyrid-5-yl or 2-chlorothiazol-5-yl; R and R3 independently are hydrogen or C¿1?-C4alkyl; R?1 and R2¿ independently are hydrogen or C¿1?-C4alkyl or R?1 and R2¿ are taken together with the nitrogen atoms to which they are attached to form an imidazoline or an oxadiazine ring; and X is N-NO¿2? or N-CN. Also claimed is a compound of formula (IA) and protein conjugate of formula (II) in which PR is a protein residue of a carrier molecule selected from the group consisting of a purified protein derivative (PPD, Tuberculin) from Diptheria virus, bovine serum albumin, cationized bovine serum albumin, human serum albumin, ovalbumin and keyhole limpet hemocyanin; or an enzyme residue selected from the group consisting of alkaline phosphatase, horseradish peroxidase, and β-galactosidase.</p> |
