发明名称 |
Generation of single-strand circular dna from linear self-annealing segments |
摘要 |
The present invention provides a method for the rapid simultaneous production of a plurality of single-stranded DNA circles having a predetermined size and nucleotide sequence using pre-designed hairpin oligonucleotides containing complementary sequences for directing ligation to form dumbbell-shaped monomers followed by heat denaturation to yield single-stranded DNA circles. |
申请公布号 |
AU1804001(A) |
申请公布日期 |
2001.06.12 |
申请号 |
AU20010018040 |
申请日期 |
2000.11.28 |
申请人 |
MOLECULAR STAGING, INC. |
发明人 |
PATRICIO ABARZUA |
分类号 |
C12N15/09;C12N15/10;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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