IMPROVED FLOW CYTOMETER NOZZLE AND FLOW CYTOMETER SAMPLE HANDLING METHODS

摘要

A method of flow cytometry sample processing, comprising the steps of establishing a sheath fluid; injecting a sample into said sheath fluid at an injection point; subjecting said sample to a first axial motion surface in a nozzle; transitioning to a second axial motion surface in said nozzle; subjecting said sample to said second axial motion surface in said nozzle wherein said first and said second axial motion surfaces transition with a maximal acceleration differentiation; coordinating said maximal acceleration differentiation so as to not exceed the practical capabilities of said sample over its length; affirmatively limiting said maximal acceleration differentiation so as to not exceed the practical capabilities of said sample over its length; exiting said sample from said nozzle; analyzing said sample.