GENERATION OF SINGLE-STRAND CIRCULAR DNA FROM LINEAR SELF-ANNEALING SEGMENTS

摘要

The present invention provides a method for the rapid simultaneous productio n of a plurality of single-stranded DNA circles having a predetermined size an d nucleotide sequence using pre-designed hairpin oligonucleotides containing complementary sequences for directing ligation to form dumbbell-shaped monomers followed by heat denaturation to yield single-stranded DNA circles.