New nucleotide sequences coding for the glk-gene.

摘要

Isolated polynucleotide (I), from coryneform bacteria, is: (i) at least 70% identical with a sequence encoding a 323 amino acid (aa) polypeptide (S1) given in the specification; (ii) encodes a polypeptide containing a sequence at least 70% identical with (S1); (iii) complementary to (i) or (ii); or (iv) contains at least 15 consecutive nucleotides (nt) of (i)-(iii). Independent claims are also included for the following: (1) vector (II) containing (I); (2) coryneform bacterium containing (II); and (3) production of L-aa by fermenting a coryneform bacterium in which at least the glucokinase-encoding gene (glk) is amplified, especially overexpressed.