Apparatus for the production of TGF-beta

摘要

Transforming growth factor beta (TGF- beta ) is produced in relatively large quantities and at a relatively high purity by fermentation in a perfusion microcarrier reactor. Conditioned media from the reactor is first treated to provide the active form of TGF- beta and subsequently purified by cation exchange chromatography followed by hydrophobic interaction chromatography. Optionally, nucleic acids complexed with the TGF- beta may be removed while the protein is bound to the cation exchange resin. Purified TGF- beta I compositions have a specific activity above 10<7> U/ml. <IMAGE>