发明名称 |
Transgenic organisms having tetracycline-regulated transcriptional regulatory systems |
摘要 |
Transgenic animals carrying a transgene comprising a nucleic acid molecule encoding protein useful for regulating the expression of genes in eukaryotic cells and organisms in a highly controlled manner are disclosed. In the regulatory system of the invention, transcription of a tet operator-linked nucleotide sequence is stimulated by a transcriptional activator fusion protein composed of two polypeptides, a first polypeptide which binds to tet operator sequences in the presence of tetracycline operatively linked to a second polypeptide activates transcription in eukaryotic cells. In a preferred embodiment, the transgene encoding the transcriptional activator fusion protein is integrated at a predetermined location within the chromosome of the transgenic animal.
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申请公布号 |
US6242667(B1) |
申请公布日期 |
2001.06.05 |
申请号 |
US19980161902 |
申请日期 |
1998.09.28 |
申请人 |
BASF AKTIENGESELLSCHAFT;KNOLL AKTIENGESEKKSCGAFT |
发明人 |
BUJARD HERMANN;GOSSEN MANFRED |
分类号 |
A01K67/027;A61K48/00;C07K14/00;C07K14/035;C07K14/195;C07K14/245;C07K19/00;C12N15/11;C12N15/62;C12N15/63;C12N15/67;C12N15/85;(IPC1-7):C12N15/09;C12N15/82;C12N15/87;C12N15/90;A01H5/00 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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