发明名称 |
POLYNUCLEOTIDE LIGANDS AS ANTI-VIRAL AGENTS |
摘要 |
Infectious human cytomegalovirus (HCMV) were isolated <i>in vitro</i> from a pool of randomized sequences after sixteen or 21 cycles of selection and amplification. The ligands characterized exhibited high HCMV-binding affinity in <i>in vitro</i> and effectively inhibited viral infection in tissue culture. Several ligands blocked viral entry. Their antiviral activity was also specific as the ligands only reacted with strains of HCMV, but not with the related herpes simplex virus 1 and human cells. Moreover, the ligands recognize several different epitopes. Thus, RNA ligands can function to bind to a human virus and block viral infection. The screening method may utilize the novel features of binding to intact infectious virus, partitioning the bound polynucleotide from unbound by passing through a porous filter, and enhancing the release of bound polynucleotides by treatment with protease.
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申请公布号 |
WO0138341(A1) |
申请公布日期 |
2001.05.31 |
申请号 |
WO2000US32259 |
申请日期 |
2000.11.21 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
LIU, FENYONG;WANG, JUN;JIANG, HONG |
分类号 |
A61K31/70;(IPC1-7):C07H21/02;C12Q1/20 |
主分类号 |
A61K31/70 |
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