| 摘要 |
This invention relates to a method of analysing a sample (21) in Raman spectroscopy, the method comprising tuning the molecules of the sample (21) with monochromatic light, collecting and analysing the light that has Raman scattered from the sample (21) with a spectrometer (25). The method comprises using a cover slip (22) below an objective (24), filling the space between the objective and the cover slip (22) with a first immersion liquid (28) and the space between the cover slip (22) and the sample (21) surface with a second immersion liquid (27), and selecting said second immersion liquid (27) so that the refractive index of the second immersion liquid (27) is substantially the same as the refractive index of the sample (21) to be analysed. |
