Methods for generating polynucleotides having desired characteristics by iterative selection and recombination

摘要

A method of producing a recombined polynucleotide having a desired characteristic, comprising:
(a) providing a population of single-stranded nucleic acid fragments corresponding to a set of related-sequence polynucleotides, wherein the set of related-sequence polynucleotides comprises polynucleotides with non-identical sequences;(b) providing at least one template polynucleotide wherein the population of single-stranded fragments is capable of hybridizing to the at least one template polynucleotide;(c) hybridizing the population of single-stranded fragments to the at least one template polynucleotide and extending the hybridized fragments on the at least one template polynucleotide with a polymerase, thereby forming a plurality of sequence-recombined polynucleotides, said sequence-recombined polynucleotides comprising sequences from the set of related-sequence polynucleotides and sequences derived from the at least one template polynucleotide; and,(d) selecting or screening the sequence-recombined polynucleotides for the desired characteristic.