METHOD AND APPARATUS FOR PREDICTING PROTEIN FUNCTION SITE, METHOD FOR IMPROVING PROTEIN FUNCTION, AND FUNCTION-IMPROVED PROTEIN

摘要

The present application provides a method for predicting the functional site of a protein using data of the entire proteins of an organism of which genome data or cDNA data is known, comprising the steps of calculating the frequency of occurrence of an oligopeptide in the entire proteins, calculating the value of each amino acid residue contributing to the frequency of occurrence as the representative value of the function, and predicting the protein functional site by using the representative value of function as an indicator. The present invention also provides a system for predicting a functional site for automatically performing said methods. Additionally, the present application provides a method for preparing a function-modified protein comprising subjecting the amino acid residues composing the functional site identi fiedby the method described above to artificial mutation, and a novel thermophilic DNApolymerase prepared by the method.