发明名称 |
METHOD FOR LOWERING POLLEN FERTILITY WITH GENE OF POLLEN- SPECIFIC ZINC FINGER TRANSCRIPTION FACTOR |
摘要 |
PROBLEM TO BE SOLVED: To provide a genetic engineering method useful for the modification of vegetable characters represented by male sterility by using a gene specific to the tissue of anther. SOLUTION: The objective genetic engineering method comprises a step to prepare a vegetable expression cassette containing a nucleic acid selected from (i) a DNA having a specific base sequence, (ii) a DNA hybridizing with the DNA having the base sequence (i) under stringent condition and coding for a transcription factor controlling the development of pollen and (iii) a DNA consisting of a fragment of the DNA (i) or (ii) and a promoter connected to the nucleic acid in actionable state, a step to produce a vegetable cell having an endogenous transcription factor controlling the development of pollen wherein the gene coding for the endogenous transcription factor is hybridized with the nucleic acid under stringent condition, a step to transduce the expression cassette into the vegetable cell, a step to grow the vegetable cell to a vegetable and a step to select a vegetable having suppressed expression of the endogenous transcription factor wherein the DNA (ii) does not contain the DNA coding for the transcription factor controlling the development of pollen. |
申请公布号 |
JP2001145430(A) |
申请公布日期 |
2001.05.29 |
申请号 |
JP19990330681 |
申请日期 |
1999.11.19 |
申请人 |
NATL INST OF AGROBIOLOGICAL RESOURCES;BIO ORIENTED TECHNOL RES ADVANCEMENT INST |
发明人 |
KAPUR SANJAE;KOBAYASHI AKIRA;TAKATSUJI HIROSHI |
分类号 |
C12N15/09;A01H5/00;C12N5/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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