| 摘要 |
PURPOSE: To provide a method for determining the peak of the wave form for the determination of a base sequence, capable of extracting a substantially true wave form, even when the wave form signal of fluorescent strength per time (occurrence frequency) contains connected waves. CONSTITUTION: The left and right sides of a wave form peak are grasped as a Gaussian distribution and a Cauchy distribution, respectively, and the wave form peak is thus grasped as the peal having different wave form characteristics on the left and right sides, respectively. The half-value widths of the left and right sides are computed, respectively, and used as barometers. A base having wave form data little in the fluctuation of the position of the peak is first extracted as a normal wave form from data comprising the computed half-value widths and the strength of the peak of each wave form length. The pitch ΔTn of the wave form is determined on the basis of the data of the wave signals of the normal wave form for each interval. The strength I and position of the peak of a true base signal peak in continuous connected wave form signals not selected as the base of the true base signal are determined from the above-described equation and the above-described value: ΔTn. |
