发明名称 |
INDUCIBLE SCREEN FOR DRUG DISCOVERY |
摘要 |
A method for identifying a polynucleotide, the expression of which is modulated in the presence of nitric oxide (NO), which method comprises: (i) providing an mRNA or cDNA population from cells which contain a polynucleotide construct, which construct comprises: (a) a promoter operably linked to a coding sequence, wherein the promoter is responsive to ecdysone or an analog thereof and the coding sequence encodes a nitric oxide synthase (NOS) or a functional variant thereof; or (b) a promoter operably linked to one or more tetracycline operator site sequences and a coding sequence in that order, wherein the coding sequence encodes a nitric oxide synthase (NOS) or a functional variant thereof; (ii) providing an mRNA or cDNA population from cells as defined in step (i), said cells having been contacted with ecdysone or an analog thereof; and (iii) comparing the populations of steps (i) and (ii), thereby to determine which polynucleotides show modulated expression in the presence of NO.
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申请公布号 |
WO0109375(A3) |
申请公布日期 |
2001.05.25 |
申请号 |
WO2000GB02932 |
申请日期 |
2000.07.28 |
申请人 |
UNIVERSITY COLLEGE LONDON;CHARLES, IAN, GEORGE;XU, WEIMING;LIU, LIZHI |
发明人 |
CHARLES, IAN, GEORGE;XU, WEIMING;LIU, LIZHI |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;A61K38/00;C07K14/47;C12N5/10;C12N15/63 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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