摘要 |
<p>A method and apparatus for separating mixtures of amphoteric substances uses two dimensions of separation. A sample valve (8, 9) is used to introduce a sample into the mobile phase (7b) for entry into the first dimension of separation, typically a chromatography column (10). The separated sample is passed to the second dimension, which is formed by a series of stages (11, 18, 19). The first stage (11) has a manifold having an anode (13), cathode (12) and a membrane (15) of a specific pK, defining two channels (16a, 16b). The amphoteric molecules are segregated between the two channels and the segregated molecules are passed to a second stage, where each channel (16a, 16b) is further split into two channels (16e, 16f and 16c, 16d), respectively. In each subsequent stage (19), the number of channels is doubled from the previous stage, increasing the degree of separation by pH.</p> |