发明名称 |
SELECTABLE CELL SURFACE MARKER GENES |
摘要 |
The present invention concerns a method of identifying genetically modified mammalian cells using a mutated protein-tyrosine kinase receptor (PTKR) as a selectable marker in mammalian cells. Particularly preferred mutated PTKR selective markers are mutated epidermal growth factor receptor (EGFR) family members, and muscle specific tyrosine kinase receptor (MuSK-R) family members. Further a method for the immunoselection of transduced mammalian cells is disclosed comprising retrovirally transducing mammalian cells with a nucleic acid sequence encoding a mutated EGFR, incubating the transduced cells with a marked antibody which recognizes and binds specifically to the mutated PTKR, and identifying the marked transduced cells.
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申请公布号 |
WO0136659(A2) |
申请公布日期 |
2001.05.25 |
申请号 |
WO2000EP11474 |
申请日期 |
2000.11.17 |
申请人 |
NOVARTIS AG;NOVARTIS-ERFINDUNGEN VERWALTUNGSGESELLSCHAFT M.B.H;PIPPIG, SUSANNE, DAGMAR;VERES, GABOR |
发明人 |
PIPPIG, SUSANNE, DAGMAR;VERES, GABOR |
分类号 |
G01N33/50;C07K14/705;C12N9/12;C12N15/09;C12Q1/02;G01N33/15;G01N33/53;G01N33/566;G01N33/569;(IPC1-7):C12Q1/00 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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