发明名称 |
Nucleotide sequences coding for the export of branched-chain amino acids, process for the isolation thereof and use thereof. |
摘要 |
Isolated polynucleotide (I) contains at least one sequence that (i) is 70% identical with a sequence that encodes at least one of 251 amino acid (aa) (3) or 108 aa (5) polypeptides; (ii) encodes a polypeptide at least 70% identical with (3) or (5); (iii) is the complement of (i) or (ii), or (iv) contains at least 15 consecutive bases from (i)-(iii). Independent claims are also included for the following: (a) protein derived from sequences of 753 bp (2) or 324 bp (4); (b) coryneform microorganisms, especially Corynebacterium, transformed with one or more (I), where these are replicative DNA; (c) production of branched-chain L-aa by fermentation of coryneform bacteria in which the brnE and/or brnF genes (or equivalent sequences) are amplified, especially overexpressed; and (d) method for isolating the brnE and/or brnF genes. |
申请公布号 |
ZA200006041(B) |
申请公布日期 |
2001.05.18 |
申请号 |
ZA20000006041 |
申请日期 |
2000.10.26 |
申请人 |
DEGUSSA-HUELS AKTIENGESELLSCHAFT;FORSCHUNGSZENTRUM JUELICH GMBH. |
发明人 |
NICOLE KENNERKNECHT;LOTHAR EGGELING;HERMANN SAHM;WALTER PFEFFERLE |
分类号 |
C12N15/09;C07K14/34;C12N1/21;C12N15/31;C12N15/54;C12P13/06;C12P13/08;C12R1/15 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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