摘要 |
<p>Disclosed are a method and apparatus for determining organic substances, namely, proteins (42) and associated cancer cells (40') in a sample (4a). A plurality of fluorescent materials (40) being selected for coupling to a specific type of substance (42) of interest, is deployed in the tissue sample. The sample (4a) is illuminated with the excitation light (14a'-14c'), which causes any of the fluorescent materials (40) in the sample (4a), to emit their characteristic wavelengths. The emitted characteristic wavelengths, indicating that at least one of the substances (42) is present within the sample (4a), are detected. The invention enables the sample (4a) to be analyzed for the presence of multiple types of proteins (42) and associated cancer cells (40') simultaneously. The invention also enables the proteins (42) and cancer cells (40') to be identified using a spectral and temporal analysis. These identifications are made regardless of whether or not the fluorescent materials (40) emit similar or different wavelengths.</p> |