The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous 5nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
申请公布号
WO0030437(A9)
申请公布日期
2001.05.17
申请号
WO1999US26848
申请日期
1999.11.17
申请人
WISCONSIN ALUMNI RESEARCH FOUNDATION;BREMEL, ROBERT, D.;BLECK, GREGORY, T.