| 摘要 |
<p>Mouse PCLP1 is identified by expression cloning with the use of a monoclonal antibody against a surface antigen of a cell line originating in mouse AGM. By fractionating PCLP1-positive/CD45-negative cells and culturing in vitro it is clarified that these cells differentiate into endothelioid cells, angioblast-like cells and hematopoietic cells. By transferring the PCLP1-positive/CD45-negative cells into a mouse defective in the hematopoietic function, the hematopoietic system is reconstructed over a long time. These facts indicate that the PCLP1-positive/CD45-negative cells might contain mammalian hemangioblasts capable of expressing the activity as long terminal reconstructive hematopoietic stem cells (LTR-HSC). A method of preparing a cell fraction containing hemangioblasts, the cell fraction prepared by this method and use of this cell fraction.</p> |
