AMINO ACID SEQUENCE OF L-PHENYLALANINE AMMONIA-LYASE, STRUCTURAL GENE THEREOF, NOVEL DNA SEQUENCE CONTAINING SAME, NOVEL RECOMBINANT DNAPLASMID CONTAINING SAME TRANSFORMANT FORMED THEREWITH, AND PROCESS FOR THE PRODUCTION OF L-PHENYLALANINE BY USING SAME

摘要

The structural gene for L-phenylalanine ammonialyase (PAL) and the amino acid sequence of PAL have been elucidated in Rhodosporidium toruloides, and novel recombinant DNA plasmids (e.g., pSW101, pYtrp6 and pKY201) have been created by inserting a DNA strand coding for the PAL gene between the 3'-terminus of the promoter region and the 5'-terminus of the terminator region. Moreover, transformants having such a novel recombinant DNA plasmid have been created, and a process for the production of PAL by growing such a novel transformant so as to cause PAL to be produced and accumulated in the culture has been established. PAL is useful in the production of L-phenylalanine, an essential amino acid used in the manufacture of artificial sweeteners. There is disclosed a novel technique for the production of L-phenylalanine by reacting an ammonia donor with cinnamic acid in the presence of the PAL prepared by the aforesaid novel process.