Assay system for anti-stress agents

摘要

Methods and compositions are provided for screening compounds for anti-stress activity. A rat hepatoma-derived cell line is employed, which is particularly responsive to mitochondrial function inhibiting agents, demonstrated by the extracellular release of lactate dehydrogenase and the formation of lactate. Cytoprotectant agents are shown to reverse the lactate dehydrogenase release in the presence of mitochondrial function interfering agents, as a model for the effects of hypoxia on cellular energetic function.