| 摘要 |
Nucleotide sequences obtained from the genome of the capsid gene of FCV strain 2280 wherein the splicing sites have been modified, so as to be deleted or inactivated are provided. The nucleotide sequences include DNA sequences that encode polypeptides, such as the capsid gene of FCV strain 2280, whereby the coding sequences are capable of being transcribed in the nucleus of a eukaryotic organism without the DNA coding sequence being altered by the organism's natural splicing machinery and the amino acid structure of the expressed protein is not altered. The recombinant molecules may be incorporated into FHV-1 vectors that are used to infect cell cultures for use, inter alia, in the development of vaccines and, in particular vaccines for preventing or treating FCV disease. Also provided are methods for obtaining nucleotide sequences coding for polypeptides that are naturally transcribed in the cytoplasm of cells and, in particular, DNA sequences coding for the FCV capsid protein, which sequences are modified so as to permit the transcription thereof in the nucleus of eukaryotic cells without the modified coding sequence being altered by the eukaryotic cells' splicing machinery.
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