Methods to screen microorganisms or gene libraries for products secreted from a cell

摘要

The invention describes methods for screening for products secreted from the cells, and provides methods to establish a correlation between the activity of the secreted product and the secreting cell. Accordingly in a first aspect the present invention relates to a method for screening a DNA library for DNA of interest comprising the steps of a) creating host cells comprising the DNA library, b) generating samples each comprising a host cell of step a), c) establishing a means for correlating host cell secretion of a material of interest in a sample with the fluorescence of the sample, d) determining which intensity interval of fluorescence indicates secretion in the sample when the correlating means of step c) is used, e) cultivating the samples under suitable conditions, and f) selecting the samples exhibiting fluorescence within the intensity interval of step d) using a fluorescence analyzer; wherein the host cell comprises DNA of interest.