| 摘要 |
<p>The present invention relates to a recombinant yeast strain for expressing human lactoferrin, N-lobe lactoferrin, C-lobe lactoferrin and process for preparation recombinant human lactoferrin, N-lobe lactoferrin, C-lobe lactoferrin using the same. The process comprises preparing recombinant vector pPICLF, pPICLFN and pPICLFC respectively for expressing human lactoferrin, N-lobe lactoferrin and C-lobe lactoferrin genes by amplifying human lactoferrin, N-lobe lactoferrin and C-lobe lactoferrin genes using the PCR method and inserting them to vector pPIC9K; obtaining recombinant yeast strain Pichia pastoris LF2(KCCM-10211), Pichia pastoris NFL3(KCCM-10210) and Pichia pastoris CLF3(KCCM-10212) by transforming auxotroph Pichia pastoris GS115 deficient in histidine dehydrogenase activity using said recombinant vectors; and mass-producing recombinant human lactoferrin, N-lobe lactoferrin and C-lobe lactoferrin by culturing the said recombinant yeast strains.</p> |
