发明名称 |
RECOMBINATION PRODUCTION OF HOLO-CITRIC ACID LYASE |
摘要 |
PROBLEM TO BE SOLVED: To provide a recombinant protein which is soluble and active and has an improved citric acid lyase activity. SOLUTION: This method for producing a protein having a citric acid lyase activity comprises expressing a proper plasmid in a host organism and then isolating the protein. Therein, the plasmid contains an inducing promoter and information originated from a gene cluster comprising at least six kinds of genes. |
申请公布号 |
JP2001120285(A) |
申请公布日期 |
2001.05.08 |
申请号 |
JP20000300878 |
申请日期 |
2000.09.29 |
申请人 |
ROCHE DIAGNOSTICS GMBH |
发明人 |
BOTT MICHAEL;DIMROTH PETER;SCHNEIDER KARIN |
分类号 |
C12N15/09;C12N9/00;C12N9/88;C12N15/60;C12Q1/32;C12Q1/527;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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