Enzymatic substrate, synthesis method and uses

摘要

The invention concerns novel enzymatic substrates of general formula (I) wherein: X represents a nitrogen atom or a carbon atom substituted with a phenyl group, said phenyl group being optionally substituted in meta or para position: Y and Z represent a hydrogen atom when X represents a carbon atom or Y and Z represent together an ether, thioether or amine bond optionally substituted with an alkyl or aryl group; R₁ and R₂ independently represent each H, Cl, F, I, Br and can be identical or different or R₁ and R₂ together represent a substituted or non-substituted fused benzene ring, R₃ and R₄ independently represent each H, Cl, F, I, Br and can be identical or different; R represents a group such that the O-R bond is capable of being hydrolysed by an enzyme. The invention also concerns a method for synthesizing said substrates, a composition, a kit and a method for detecting at least one micro-organism using said enzymatic substrate.