REACTION METHOD, REACTION APPARATUS AND ENZYME

摘要

A reaction method wherein a mediator in an activated state is poured into a reaction system containing a substrate. In this method, it is preferable to activate the mediator under mild conditions with the use of an enzyme as a preliminary step. It is particularly preferable that a highly active mediator, which has been improved in the activity and/or stability, is poured into the above-described reaction system. A method of enzymatically decomposing a substrate comprising immobilizing an oxidizing enzyme peroxidase in a structural unit which has almost the same size as the enzyme size and a structural stability, and decomposing the substrate in the presence of an oxidizing agent at such a concentration as being accepted by the immobilization. A reaction promoter which is to be used in a reaction system of decomposing a substrate with an oxidizing enzyme, has a diketone structure allowing radicalization due to the function of the oxidizing enzyme and is soluble in water. A manganese peroxidase liquor and manganese peroxidase obtained by culturing a white rot fungus variant SC26 strain.