DETECTION OF MICROORGANISMS

摘要

The present invention relates to a medium for the detection of Salmonella, Shigella and E. coli 0157 species, the selective media comprising a growth nutrient base incorporating growth substrates for E. coli 0157 and Shigella, sugar fermentable by E. coli species other than E. coli 0157, bile salts, citrate, magnesium ions and calcium ions in amounts such that the media allows growth of E. coli 0157, Shigella and Salmonella whilst inhibiting growth of other bacteria; an H2S substrate for detecting hydrogen sulphide production; a chromogenic substrate for detecting beta -galactosidase activity; and an indicator substrate for detecting fermentation of the sugar of (ii) and the use of the medium to detect Salmonella, Shigella and E. coli 0157 species in clinical or food or water samples.