发明名称 |
FLUORESCENT CAPTURE ASSAY FOR KINASE ACTIVITY |
摘要 |
A method for determining the level of tyrosine kinase activity in a biologic al sample is disclosed. The method employs an anti-phosphotyrosine antibody as both the capture agent and the detecting agent. The detecting antibody is labeled with a fluorescent label, for instance, Cy5,Cy5.5 or Cy7 or a lanthanide ion, such as europium, as the signal generating entity. The methodis particularly well suited to high throughput screening, for example, for compounds which modulate tyrosine kinase activity.
|
申请公布号 |
CA2388186(A1) |
申请公布日期 |
2001.05.03 |
申请号 |
CA20002388186 |
申请日期 |
2000.10.23 |
申请人 |
PHARMACOPEIA, INC. |
发明人 |
INGLESE, JIM;WEBB, MARIA L.;DAMAJ, BASSAM;GLICKMAN, J. FRASER;BURBAUM, JONATHAN J. |
分类号 |
C12Q1/48;G01N33/573;(IPC1-7):G01N33/53;A61K31/445;C07K1/13;G01N33/543 |
主分类号 |
C12Q1/48 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|