摘要 |
The invention concerns a method for continuously obtaining two-dimensional microcarrier beads (2D-MS) displaying particular functional characteristics for anchorage-dependent cell culture (CAD), comprising a succession of steps which consist in: continuously producing polymer film rolls from a specific polymer granules; activating said polymer film by any means generating reactive groups; covalent grafting between the activated polymer film and a polymer, copolymer or a macromolecule of interest; if required, washing to eliminate the monomers which have not been consumed and fixed on the film, followed by drying the grafted film; and finally cutting the grafted polymer film by continuous punching, the size of the punch being selected according to that of the dimension desired for the two-dimensional microcarrier beads. |
申请人 |
"COMPUTER CELL CULTURE CENTER";MILLER, ALAIN, OTHON, ANDRE;ROPSON-KENDA, NATHALIE, LILIANE, PAULE, GHISLAINE |
发明人 |
MILLER, ALAIN, OTHON, ANDRE;ROPSON-KENDA, NATHALIE, LILIANE, PAULE, GHISLAINE |