| 摘要 |
<p>The present invention provides a novel approach to gene therapy of restricted areas such as tumors. The methods introduced here comprise: (a) placing a gene of interest in a plasmid vector driven by a tissue specific promoter or a heat or light inducible promoter; and (b) modifying this vector by including a tetracycline responsive fusion protein which acts as a transcriptional activator, thus permitting regulation of gene expression by varying the levels of tetracycline; (c) modifying this vector by including DNA sequences that reduce or eliminate expression of genes in unintended cells. Also provided are a set of vectors for both sustained and regulated expression. There are also presented novel vectors for the gene therapy treatment of metastatic breast, ovarian and prostate cancer.</p> |
