发明名称 |
Thermostable enzyme promoting the fidelity of thermostable DNA polymerases-for improvement of nucleic acid synthesis and amplification in vitro |
摘要 |
A purified thermostable enzyme is derived form the thermophilic archaebacterium Archaeoglobus fulgidus. The enzyme can be native or recombinant, is stable under PCR conditions and exhibits double strand specific exonuclease activity. It is a 3'-5'exonuclease and cleaves to produce 5'-mononucleotides. Thermostable exonucleases are useful in many recombinant DNA techniques, in combination with a thermostable DNA polymerase like Taq especially for nucleic acid amplification by the polymerase chain reaction (PCR). <IMAGE> |
申请公布号 |
AU7907700(A) |
申请公布日期 |
2001.04.30 |
申请号 |
AU20000079077 |
申请日期 |
2000.09.27 |
申请人 |
ROCHE DIAGNOSTICS GMBH |
发明人 |
WALTRAUD ANKENBAUER;FRANK LAUE;HARALD SOBEK;MICHAEL GREIF |
分类号 |
C12N15/09;C12N9/12;C12N9/16;C12N9/22;C12N15/10;C12N15/54;C12N15/55;C12P19/34;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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