STABILIZED PROTEINS

摘要

The invention described herein comprises methods for stabilizing polypeptides and polypeptide complexes. The stabilization methods include controlled cross-link reaction such that polypeptides and polypeptide complexes maintain their original functionality. Embodiments of the invention outlining methods for identification of amino acid residues which when cross-linked are least disruptive to the structure and function of the polypeptides or polypeptide complex; as well as methods for mutagenesis for identifying residues to further control the cross-link reaction; and statistical analysis of the data base for the identification suitable residue pairs which are least likely to be disruptive of structure and function when cross-linked. Detailed cross-linked procedures and reaction conditions are exemplified and discussed.