| 摘要 |
<p>The invention relates to a method for the generation of repeat-depleted DNA comprising amplifying repetitive template DNA by a first polymerase chain reaction (PCR), wherein the hybridization step is a low stringency hybidization step and a second PCR followi ng the first PCR, wherein the hybridization step of said second PCR is a high stringency hybridization step. The repeat-depleted DNA obtained can be used as probe or cloned into vectors, plasmid, etc. Further, the invention relates to the application of the method in the generation of and hybridization with DNA libraries, DNA arrays or DNA blots.</p> |
