| 摘要 |
Capped RNA corresponding to the spliced transcript of the <i>Minos</i> transposable element has been synthesised <i>in vitro</i> and shown to be active as a source of transposase for <i>Minos</i> transposon mobilisation. When co-injected into <i>Drosophila</i> and Medfly embryos, <i>Minos</i> mRNA can mobilise homologous transposons from plasmids into germline chromosomes. Injected mRNA is superior to injected plasmids expressing the transposase as a source of the enzyme, resulting in several-fold increases in transformation efficiencies. In a rapid test for mRNA activity, <i>Minos</i> mRNA has been shown to catalyse excision of a <i>Minos</i> transposon from plasmid DNA in Medfly embryos. High frequency mobilisation of a <i>Minos</i> transposon from the X chromosome into autosomes has also been demonstrated after injections of <i>Minos</i> transposase mRNA into pre-blastoderm <i>Drosophila</i> embryos. The rates of transposition (approximately 30 %) suggest that this is a powerful system for genetic manipulation.
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