METHOD FOR DETERMINING LIPID AND PROTEIN CONTENT OF TISSUE

摘要

Disclosed is a method for determining lipid content and identity and protein content and identity in a tissue of a subject. In a preferred embodiment, the method comprises contacting the tissue with electromagnetic radiation having an excitation wavelength, and collecting the Raman spectra emitted by the tissue in a range of wavelengths associated with lipids and/or proteins. The method further comprises altering the temperature of the tissue, such as by heating or cooling, and repeating the collection of Raman spectra while the temperature of the tissue is altered. The method further comprises analyzing the spectra collected to determine an amount of lipid and/or protein present in the tissue. The method can also be used for determining the depth of a source of the spectra emitted by the tissue. For example, the determining can comprise using a confocal lens system to collect emitted spectra. The analyzing can further comprise determining the type of lipid or protein present in the tissue based on the depth of the source of the spectra emitted by the tissue.