| 摘要 |
<p>Methods are disclosed whereby variants of proteins which do not confer selectable phenotypes can nevertheless be selected for stable expression in heterologous hosts. Related methods are disclosed whereby cDNA expression libraries can be enriched for stable expression of autonomously folding domains in heterologous hosts. Related methods are further disclosed whereby peptides which stabilize unstable proteins may be selected from random peptide libraries. If a heterologous protein is expressed as a fusion with a selectable phenotype, the strength of the phenotype is proportional to the folding rate, and therefore the solubility of the protein of interest. Thus, the selectable phenotype can be used to select better expressors from libraries of mutagenized proteins of interest, or it can be used to select autonomously folding domains (AFD) from cDNA expression libraries, or it can be used to select peptides which stabilize unstable proteins.</p> |
