| 摘要 |
Disclosed herein is a method for potentiating antisense RNA activity, involving joining the antisense RNA to a protein-coding nucleic acid sequence. Also disclosed are nucleic acid sequences that include an antisense RNA joined to a protein-coding sequence, as well as nucleic acids which, when transcribed, generate such antisense products. Shown schematically in the accompanying figure is an engineered version of the yeast Flp site specific recombinase gene which is useful in such gene therapy vectors, whereby Flpe-mediated recombination results in expression of operably linked reporter protein upon expression of the Flpe protein in a host cell containing a Flpe substrate plasmid. |
