摘要 |
The process of the invention provides for the reconstitution of the polymerase III* subassembly, Pol III*, of E. coli DNA polymerase III holoenzyme from substantially pure peptide subunits. In the first of two general schemes in which the subunits are added in a specified order, gamma and &tgr; are premixed before addition of delta and delta'. In the second general scheme, delta' is first assembled onto gamma (or &tgr;); then the excess delta' is removed before adding &tgr; (or gamma), following which delta is added. Reconstituted Pol III* had the same subunit composition as purified natural Pol III*, as well as similar activity. Other smaller subassemblies of the polymerase III holoenzyme may also be reconstituted by the process of the invention.
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