摘要 |
In a method for characterizing a culture liquid, in particular in a bioreactor, by illuminating the cells ( 22 ) contained in the culture liquid the culture liquid is analyzed in situ, microscopic imaging of the cells ( 22 ) and by evaluating the image. The illumination is carried out as dark-field illumination, and the image evaluation comprises a comparison of the intensities of the light output by the interior of the cell and by the edge of the cell, by means of which comparison living and dead cells ( 22 ) are distinguished in order to determine the vitality of the culture liquid. |