发明名称 |
PURIFICATION OF RECOMBINANT PROTEINS FUSED TO MULTIPLE EPITOPES |
摘要 |
The present invention provides novel identification polypeptides containing multiple copies of an antigenic domain joined in tandem to provide increased sensitivity for the detection and purification of target peptides, a cleavab le linking sequence and optionally a spacer domain. Further provided are hybrid polypeptide molecules composed of an identification polypeptide and a target peptide which are produced by recombinant DNA technology and purified using affinity chromatography using one or more ligands. Accordingly, also provide d are DNA expression vectors containing DNA encoding for identification polypeptides and methods for using such identification polypeptides for the purification of target peptides. Also provided are methods of constructing D NA vectors encoding the novel identification polypeptides and DNA expression vectors encoding the identification polypeptides linked to a target peptide.
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申请公布号 |
CA2386471(A1) |
申请公布日期 |
2001.04.19 |
申请号 |
CA20002386471 |
申请日期 |
2000.09.20 |
申请人 |
SIGMA-ALDRICH CO. |
发明人 |
BRIZZARD, BILL;HERNAN, RON |
分类号 |
C12N15/09;C07K1/22;C07K14/00;C07K19/00;C12N15/62;C12P21/02;(IPC1-7):C12N15/62;C12N9/16;G01N33/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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