Tenocyte compositions, preparation and use

摘要

Composition (A) comprising human, autologous and/or allogeneic tenocytes (TC). Independent claims are also included for the following: (1) composition comprising genetically modified human or equine TC and an excipient; (2) isolated, genetically modified TC containing a recombinant nucleic acid (I) that encodes a growth factor and/or a factor that inhibits an inflammatory reaction; (3) composition comprising frozen TC; (4) composition (B) for preserving TC containing at least one of dimethylsulfoxide (DMSO), modified gelatin, polysaccharide or glycerol; in vitro or ex vivo genetic modification of TC by contact with naked DNA or a recombinant retrovirus pseudotyped with a GALV envelope; and (5) preparing TC comprising taking a tissue fragment, preferably human, that contains TC, dissociating TC in presence of collagenase, then in vitro cultivation and multiplication of TC by successive passaging, particularly with seeding at 1/4 of the level required for confluence.