DESIGN OF HIGH AFFINITY RNASE H RECRUITING OLIGONUCLEOTIDE

摘要

The present invention relates to the field of bicyclic DNA analogues, e.g. LNA and LNA modifications, which are useful for designing oligomers that form high affinity duplexes with complementary RNA wherein said duplexes are substrates for RNase H. The oligonucleotides may be partially or fully composed of LNA analogues with very high affinity and ability to recruit RNase H. The implications are that oxy-LNA by itself may be used to construct novel antisense molecules with enhanced biological activity. Alternatively, oxy-LNA may be used in combination with non-oxy-LNA, such as standard DNA, RNA or other analogues, e.g. thio-LNA or amino-LNA, to create high affinity, RNase H recruiting anti-sense compounds without the need to adhere to any fixed design.